Category: Heat Shock Proteins

In some T cell activation experiments, the freshly isolated PBMCs (5 105 cells) were seeded in a 24-well culture plate (1 ml/well) and stimulated by adding 2 106 Dynabeads goat anti-mouse-IgG (Invitrogen 110?33) precoated with anti-CD3 antibody (clone UCHT1, BD Biosciences, 2 g/107 beads) and anti-CD28 antibody (clone CD28

In some T cell activation experiments, the freshly isolated PBMCs (5 105 cells) were seeded in a 24-well culture plate (1 ml/well) and stimulated by […]

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